This scientific report provides an update of the Xylella spp. host plant database, aiming to provide information and scientific support to risk assessors, risk managers and researchers dealing with Xylella spp. Upon a mandate of the European Commission, EFSA created and regularly updates a database of host plant species of Xylella spp. The current mandate covers the period 2021–2026. This report is related to the 13th version of the database published in Zenodo in the EFSA Knowledge Junction community, covering literature published from 1 January 2025 up to 30 June 2025, and recent Europhyt outbreak notifications. Informative data have been extracted from 45 selected publications. One new host plant (Carya carolinae‐septentrionalis) was identified and added to the database. It was naturally infected by X. fastidiosa subsp. multiplex in the United States. No additional data were retrieved for X. taiwanensis, and no additional multilocus sequence types (STs) were identified worldwide. New information on the tolerant/resistant response of plant species to X. fastidiosa infection were added to the database. The Xylella spp. host plant species were listed in different categories based on the number and type of detection methods applied for each finding. The overall number of Xylella spp. host plants determined with at least two different detection methods or positive with one method (between sequencing and pure culture isolation (category A)), reaches now 464 plant species, 210 genera and 71 families. Such numbers rise to 728 plant species, 319 genera and 91 families if considered regardless of the detection methods applied (category E).